Method, system and device for producing signals from a substance biological and/or chemical activity

ABSTRACT

The invention concerns a method, a system and a device for producing, from a substance, electric signals characteristic of the biological activity of an active element contained in the substance. The method consists in: placing the substance in a zone subjected to a specific electric, magnetic and/or electromagnetic excitation field. The method further includes a step which consists in transforming the fields resulting form the interaction between the specific excitation filed and the substance, into signals, in particular electric signals, using a first transducer receiving the resulting fields.

The present invention relates to a method, a system and a device forproducing signals from a substance, in particular electric signals,characteristic of the biological and/or chemical activity or thebiological and/or chemical behaviour of said substance or an activeelement contained in said substance. The invention also relates to amethod and a system for controlling said signals. The invention alsorelates to the applications of said method, system and device inparticular to the production of active substances and to the detectionof defined substances. Finally, the invention relates to signals linkedto a biological and/or chemical activity thus produced by said method,system and device.

It is known from the research works of Jacques Benveniste, in particularthose described in the patent application WO 94/17406 published on Aug.4, 1994, that one can pick up, from a biological and/or chemical activeelement such as a chemical compound, a cell or a micro-organism, or froma substance containing this active element such as a purifiedpreparation, a biological sample, or a living being, an “electromagneticsignal characteristic of the biological and/or chemical activity or ofthe biological and/or chemical behaviour” of said substance and/or saidactive element contained in said substance.

It is also known that it is possible to transform, in particular bymeans of a transducer, such an electromagnetic signal into electricsignals. In the following text one also means by “electric signalscharacteristic of the biological and/or chemical activity or of thebiological and/or chemical behaviour of said substance or of an activeelement contained in said substance” the electric signals derived bysignal digitising and/or processing. In this expression the word“characteristic” is used in the meaning where the physical parameters ofthe electric signals are specific to the substance or to the activeelement contained in said substance and that the application of theseelectric signals, via a transducer, to a biological control system makesit possible:

(i) to induce a biological and/or chemical activity on said biologicalcontrol system relative to that of the substance of origin or the activeelement it contains;

(ii) to reveal a characteristic of the substance or the active elementit contains, at the origin of said electric signals.

The patent application WO 94/17406 published on Aug. 4, 1994, describesa method and a device for picking up “an electromagnetic signalcharacteristic of a biological and/or chemical activity or of abiological and/or chemical behaviour” from a biological and/or chemicalactive element such as a chemical compound, a cell or micro-organism, or from a substance containing this active element such as a purifiedpreparation, a biological sample, or a living being.

Since then the inventors have discovered that it is possible to improvethe quality of the electromagnetic signal picked up as well as thereliability of the method for producing these signals and thatconsequently it is possible to produce characteristic electric signalsappropriate for industrial applications. The production of suchcharacteristic electric signals implies an exceptional industrialimportance.

It thus becomes possible to detect and characterise active elementspresent in low concentration or in very low concentration in asubstance. As examples, it is thus possible to monitor the presence orabsence of chemical compounds such as caffeine, ionophoretic-calcium,ovalbumin, propranolol or micro-organisms such as bacterium coli,streptococci, staphilocci whose presence is looked for.

It thus becomes possible to carry out remote tests at several thousandsof kilometers since the characteristic signals are electric signalswhich can immediately be transmitted to the investigation centre of thecontrol laboratory.

It is possible to modify the biological and/or chemical activity or thebiological and/or chemical behaviour of a biological receptor system bysubmitting it to the effects of characteristic electric signals. It alsobecomes possible to produce new drugs such as solutions depending onsignals from arnica, bradykinin, caffeine, nicotine. New productiontechniques for drugs can be implemented. For example, in the case ofcertain drugs such as antibiotics, anti-viruses, anti-parasites,anti-mitotics which, to act within bacteria, viruses or cells (tumourcells in particular), must breach the defensive barriers of the above,the signals of these drugs are applied directly into the heart of thebacteria, viruses or cells. In fact, the application of characteristicelectric signals, via a n appropriate transducer, generates magneticfields which penetrate into the bacteria, viruses or cells and modifytheir chemical and/or biological behaviour.

It is possible to store the characteristic electric signals in databanks, using computer techniques. Then, the spread of therapeuticresources, from one point to the other on the planet, is instantaneousaccording to needs.

The examples described above concern the medical domain. The chemicalindustry also, such as electronic components, will also be concerned bythe new possibilities offered by the present invention. The use ofelectromagnetic fields, emitted by characteristic electric signals, tomodify the behaviour of molecules and promote chemical reactions willopen up new prospects concerning both the conception of new materialsand their methods of production. Thus, for example, it will be possibleto use them as catalysts able to influence the stereochemistry ofmolecules.

The method according to the invention making it possible to improve theperformances of characteristic electric signals comprises the stages:

of placing said substance in a zone submitted to a specific excitationfield of electric, magnetic and/or electromagnetic nature,

of transforming the fields resulting from the interaction of thespecific excitation field and the substance, into signals, in particularelectric signals, by means of a first transducer receiving saidresulting fields.

In fact, the inventors have noted that, in a surprising manner, the useof an excitation field such as for example an electromagnetic field ofuniform power spectral density over a frequency spread (for examplewhite noise of 1 Hz to 20 kHz) makes it possible to improve theperformance of characteristic electric signals. As an example of such afirst transducer, one can mention very sensitive small copper wirebobbins with an impedance of 300 Ohms; internal diameter of 6 mm,external diameter of 16 mm, length 6 mm, normally used as telephonereceivers.

Preferably, the process according to the invention further comprises thestage for processing said signals derived from said first transducer,relative to second signals derived from a second transducer receivingthe specific excitation field, in the absence of said substance. As anexample, the processing can consist of subtracting these two signals byusing two receiver bobbins connected in series and with opposite phases,one facing said substance and receiving the electromagnetic fieldthrough said substance and the other receiving the electromagnetic fielddirectly. Thus, the part of the signals really characteristic of thebiological and/or chemical activity or of the biological and/or chemicalbehaviour of said substance or said active element contained in saidsubstance, is enhanced relative to that derived from the firsttransducer alone.

As an example, according to another embodiment of the invention, theprocessing can consist of recording consecutively the signals comingfrom said substance and then the signals coming from a neutral substance(water or physiological serum), then subtracting the first signals fromthe second (which serve as reference), this subtraction being carriedout before or after processing the signals as described below(subtraction of amplitudes or power spectral densities).

Preferably, according to another embodiment of the invention, theprocess according to the invention comprises the stage of processing thesignals derived from said first transducer, in function of thecharacteristics of the specific excitation field. For example, thesignal processing consists of calculating the power spectral densityusing a Fourier transform, to narrow the useful frequency band (bandpassfilter), to normalise the specific excitation field relative to thepower spectral density, and to reconstitute a signal using an inverseFourier transform. As in the case of the preceding embodiment, the partof the signals which are really characteristic of the biological and/orchemical activity or of the biological and/or chemical behaviour of saidsubstance or said active element contained in said substance, are thusenhanced relative to that produced without processing.

Preferably, the specific excitation field has the characteristic ofhaving a uniform power spectral density over a frequency band. As anexample, the power spectral density is uniform over a frequency bandfrom 1 Hz to 20 kHz. Thus, said substance is submitted to a neutralexcitation field of the white noise type.

Preferably, furthermore, the zone submitted to the specific excitationfield is insulated from parasitic fields from the environment.

The invention also relates to the applications of the signals produced.To this effect, the method further comprises the stage of applying saidsignals from said first transducer to a biological receiver, by means ofa third transducer. In the case where said signals are processed, it isthe signals processed in this way which are applied to the biologicalsystem receptor.

As an example, said third transducer will generate and emit anelectromagnetic field in the direction of biological system receptorssuch as a carrier substance or a reactive medium producingstereochemical molecules. This electromagnetic field will modify thebiological and/or chemical activity or the biological and/or chemicalbehaviour of the biological system receiver as a function of the natureof biological and/or chemical activity or the biological and/or chemicalbehaviour of said substance. Thus, for example, it is possible to sendthe message for caffeine into a water-based beverage to produce adietetic drink or an alimentary supplement.

The invention also concerns the control of characteristic electricsignals. For this, the process further comprises the stage forcontrolling the correlation between on the one hand, the signal derivedfrom said first transducer or the processed signal and, on the otherhand, the biological and/or chemical activity or the biological and/orchemical behaviour of said substance or said active element contained insaid substance. This control is carried out by applying, by means ofsaid third transducer, the signals derived from said first transducer toa biological control system and by verifying that said biologicalcontrol system reacts in a specific manner to the signals from saidfirst transducer. In the case where said signals are processed, it isthe signals thus processed which are applied to said biological controlsystem. The reaction of said biological control system must be relatedto the nature of the biological and/or chemical activity or thebiological and/or chemical behaviour of said substance or said activeelement contained in said substance whose signals are emitted from saidfirst transducer. As an example, in particular one can cite as abiological control system: an isolated guinea-pig heart, aligand/receptor couple in particular an antigen/antibody couple, theskin of a guinea-pig or a live rabbit which is submitted to a cutaneousinjection test, isolated or cultured cells.

Surprisingly, it was noted that the method according to the inventionfor producing characteristic signals delivers exploitable signals froman active substance whose active element can even be contained in low orvery low concentrations (less the 10⁶ moles per liter). The methodaccording to the invention can thus be applied to characterise thepresence of an active element at the trace level in a substance.

The invention also relates to a system for producing signals, inparticular electric signals, characteristic of the biological and/orchemical activity or of the biological and/or chemical behaviour of asubstance or an active element contained in said substance. Theinvention also concerns a system for implementing the properties of saidsignals. Said system comprises an emitter generating a specificexcitation field of electric, magnetic and/or electromagnetic nature ina zone where said substance is located. As an example, one can cite anemitter with the following characteristics: bobbin with internaldiameter 50 mm, length 80 mm, R=3.6 ohms, 3 layers of 112 turns ofcopper wire, field on the axis to the centre 44 Oe/A, and on the edge 25Oe/A. Said system also comprises a first transducer receiving the fieldsresulting from the interaction of said specific excitation field andsaid substance, said first transducer transforming said resulting fieldsinto signals, in particular electric signals. As an example, one cancite a transducer such as a very sensitive little bobbin of copper wirewith an impedance of 300 Ohms, of internal diameter 6 mm externaldiameter 16 mm, length 6 mm, usually used for telephone receivers. Inthe case of this example the characteristics of the electric signalsderived from the transducer are as follows: amplitude of about 200 mVcrest to crest.

Said system also comprises means of emission for applying said signalsderived from said first transducer to a biological system receptor. Asan example of such means of emission, one can cite a transducer with thefollowing characteristics: bobbin with internal diameter 50 mm, length80 mm, R=3.6 ohms, 3 layers of 112 spirals of copper wire, field on theaxis to the centre 44 Oe/A, and on the edge 25 Oe/A. Examples ofbiological receptor systems have been mentioned above.

Preferably, the system according to the invention further comprisesmeans for processing said signals derived from said first transducer, infunction of the signals derived from a second transducer receiving thespecific excitation field, in the absence of said substance. Thus saidprocessed signals are more characteristic of the biological and/orchemical activity or the biological and/or chemical behaviour of saidsubstance or said active element contained in said substance.

Preferably, according to another variant of the invention, the systemfurther comprises means for processing the signals derived from saidfirst transducer, in function of the characteristics of the specificexcitation field. In the case of this variant embodiment also, saidprocessed signals are more characteristic of the biological and/orchemical activity or of the biological and/or chemical behaviour of saidsubstance or said active element contained in said substance.

Preferably, said specific excitation field has the characteristic ofhaving a uniform power spectral density over a frequency band.

Preferably, the system according to the invention further comprisesmeans for isolating said zone from parasitic fields from theenvironment.

Preferably, the system according to the invention further comprisescontrol means for controlling the correlation between, on the one hand,the signal derived from said first transducer or the processed signaland, on the other hand, the biological and/or chemical activity or ofthe biological and/or chemical behaviour of said substance or saidactive element contained in said substance. Said control means comprisea third transducer applying the signals derived from said firsttransducer to a biological control system. In the case where the signalsare processed, it is the processed signals which are applied to thebiological control system. Said control means further comprise means forverifying that the biological control system reacts in a specific mannerto the signals derived from said first transducer, according to thenature of the biological and/or chemical activity or of the biologicaland/or chemical behaviour of said substance or said active elementcontained in said substance from which the signals derived from saidfirst transducer are issued. As an example, one can cite as biologicalcontrol system: an isolated guinea-pig heart, a ligand/receptor couplein particular an antigen/antibody couple, an injectable substanceprovoking cutaneous reactions, isolated or cultured cells.

Preferably, the system according to the invention is such that saidsubstance contains a low concentration or very weak concentration of anactive element.

The invention also relates to a device for producing signals, inparticular electric signals, characteristic of the biological and/orchemical activity or of the biological and/or chemical behaviour of asubstance or an active element contained in said substance. Said devicecomprises an emitter generating a specific excitation field of electric,magnetic and/or electromagnetic nature in a zone where said substance islocated. It also comprises a first transducer receiving the fieldsresulting from the interaction of said specific excitation field andsaid substance. Said first transducer transforms said resulting fieldsinto signals, in particular electric signals. Said signals arecharacteristic of the biological and/or chemical activity or of thebiological and/or chemical behaviour of said substance or said activeelement contained in said substance.

The device according to the invention further comprises means forprocessing said signals derived from said first transducer, relative tothe signals derived from a second transducer receiving the specificexcitation field, in the absence of said substance.

According to another embodiment variant of the invention the devicefurther comprises means for processing the signals derived from saidfirst transducer, in function of the characteristics of the specificexcitation field.

Preferably, said specific excitation field has the characteristic of auniform power spectral density over a frequency band.

Preferably, the device according to the invention further comprisesmeans for isolating said zone from parasitic fields from theenvironment.

The invention also relates to the applications of the method, system orthe device described above. More particularly, the invention concernsthe production of active substances in particular the production ofdrugs. Said active substances are produced by applying said signalsderived from said first transducer to a carrier substance. In the casewhere said signals are processed, it is the signals thus processed whichare applied to the carrier substance.

The invention also relates to the application of the process, system ordevice which has the aim of establishing a table of correlation betweenthe characteristics of a determined substance or an active elementcontained in said determined substance and the modifications they caninduce on test biological systems. Such correlation tables also enterinto the framework of the invention, as well as the use of suchcorrelation tables for detecting said determined substance or saidactive element contained in said determined substance. This detectioncan in particular be carried out remotely, after transmitting saidcharacteristic signal to a testing laboratory possessing test biologicalsystems. The correlation tables can also be used for controlling theproduction of homeopathic products, by making it possible to verify theactivity of the latter during successive phases of dilution.

The invention also relates to electric signals linked to a biologicaland/or chemical activity, obtained through implementing the method, thesystem or the device according to the invention. It is possible tocharacterise these signals from the effects they produce on a biologicalcontrol system like that described above.

Other characteristics and advantages of the invention will become clearby reading the description of the variants of embodiments of theinvention, given as indicative but non-limiting examples, and also byreading the examples of experiments making it possible to validate themethod of production of characteristic electric signals, the aim of thepresent invention, and which refer to the attached drawings in which:

FIG. 1 shows a diagram of an example of an embodiment of a system and adevice for producing characteristic electric signals, said systemcomprising an applicator making it possible to apply the characteristicsignals to a biological system receptor,

FIG. 1a shows a detailed view in perspective of a part of the device forproducing electric signals, showing the emitter of the excitation fieldand the transducer receiving the resulting fields,

FIG. 1b shows in diagrammatic form the type of micro-computer usedeither for generating the excitation fields, or for recording andtransmitting under digitised form the characteristic electric signals,or for applying the characteristic electric signals to biological systemreceivers via transducers.

FIG. 1c shows a detailed view in perspective of a part of the applicatorintended to apply the characteristic electric signals to biologicalsystem receptors,

FIG. 2 shows a drawing of an example of an embodiment of an applicatormaking it possible to control the presence of the characteristicelectric signals issued from a solution of acetylcholine by applyingthem to a biological control system constituted by an isolated perfusedguinea-pig heart,

FIG. 3 shows a drawing of an example of an embodiment of an applicatormaking it possible to apply the characteristic electric signals issuedfrom a solution containing as active biological element, Escherichiacoli K1, Streptococcus or an antibody directed against thepolysaccharidic antigen of Escherichia coli K1.

FIG. 3a shows a black and white image of 320 pixels×240 pixels ofprecipitates formed during the precipitation reaction between thepolysaccharidic antigen of Escherichia coli K1 and an antibody directedagainst this antigen, after application of characteristic electricsignals coming from a biological system containing Streptococcus,

FIG. 3b shows a black and white image of 320 pixels×240 pixels ofprecipitates formed during the precipitation reaction between thepolysaccharidic antigen of Escherichia coli KP1 and an antibody directedagainst this antigen, after application of characteristic electricsignals coming from a biological system containing Escherichia coli K1,

FIG. 3c shows a black and white image of 320 pixels×240 pixels ofprecipitates formed during the precipitation reaction between thepolysaccharidic antigen of Escherichia coli K1 and an antibody directedagainst this antigen, after simultaneous application of characteristicelectric signals coming from a biological system containingStreptococcus and coming from a biological system containing Escherichiacoli K1,

FIG. 3d shows a black and white image of 320 pixels×240 pixels ofprecipitates formed during the precipitation reaction between thepolysaccharidic antigen of Escherichia coli K1 and an antibody directedagainst this antigen, after simultaneous application of characteristicelectric signals coming from a biological system containing Escherichiacoli K1, and coming from a biological system containing a n antibodydirected against Escherichia coli K1.

FIG. 4 shows an image of the sub-cutaneous allergic reaction of a skinof a guinea-pig after injection of 0.1 ml distilled water, the distilledwater having previously been submitted to an applicator ofcharacteristic electric signals coming from a neuromediator such asacetylcholine (ACh).

Below is described an example of an embodiment of a system and of adevice for producing characteristic electric signals, with reference toFIGS. 1, 1 a, 1 b and 1 c. In these figures, a schematic drawing isgiven of a variant of an embodiment of a system making it possible toproduce characteristic electric signals and to implement them forindustrial purposes. The signals are characteristic, in the meaning ofthe present invention, of the biological and/or chemical activity or ofthe biological and/or chemical behaviour of a substance.

The system comprises a device 10 for producing electric signalscharacteristic of the biological and/or chemical activity or of thebiological and/or chemical behaviour of a substance 1 or of an activeelement contained in said substance. In the case of the variantdescribed with reference to FIGS. 1, 1 a, 1 b, 1 c, said substance 1 isa solution of caffeine 10⁻⁶ M.

The device 10, located in Paris, for example, produces characteristicelectric signals which are digitised after digital-analog conversion.The signals thus digitised are, in a known manner, transmitted remotely,for example by a computer communication network of the Internet typeusing radio links 11. The digitised signals thus transmitted arereceived by an applicator 12, located in New York for example,comprising 10 emission means 13. The emission means 13 make it possibleto apply the characteristic signals (after digital-analog conversion) toa biological system receptor. In the case of the embodiment describedwith reference to FIG. 1, 1 a, 1 b and 1 c, the biological systemreceptor is a dietetic beverage. The digitised signals can be processed27, recorded and stored 33, before their remote transmission and/orbefore having been applied to a biological system receiver.

The device for producing the signals 10 comprises a chamber 2 providedwith electric and magnetic shielding isolating it from parasitic fieldsfrom the environment. The shielded cylindrical chamber is composed ofthree superposed layers: copper, soft iron, permalloy, made from sheets1 mm thick. The chamber has an internal diameter of 65 mm, and a heightof 100 mm. The chamber is closed by a shielded lid 5. An emitter 4 issituated inside the chamber. It generates a specific excitation field ofelectromagnetic nature. The emitter is supplied by a generator, 14. Inthe chamber 2 is placed a glass container 3 with the dimensions 10 mm×10mm×4.5 mm. This container 3 holds 1 ml of the substance 1. The emitter 4comprises a bobbin advantageously completed by a magnetic core in softiron. The emitter bobbin 4 has an impedance of 300 ohms, an internaldiameter of 6 mm, an external diameter of 16 mm, and a length of 6 mm.The magnetic core in soft iron is placed in contact with the externalwalls of the container 3. Said substance is thus submitted to anexcitation field emitted by the emitter 4. The generator 14 is designedto generate a low frequency signal especially square or sinusoidal lowfrequency signals, of pink noise or, advantageously, white noise. Thespectrum of the excitation signal supplying the emitter bobbin 4corresponds closely to the spectrum of audible frequencies (20 Hz-20,000Hz). The generator 14 can be a generator of an analog signal of knowntype, using for example a read-only memory (ROM, PROM, EPROM, EEPROM)containing the digital signal of the desired noise. This memory islinked in a known way to a digital-analog converter. A microcomputer 14can also b e used, provided with a sound card 25 comprising adigital-analog converter 41. For example, one can use a computer 14 ofthe PC type, operating under the WINDOWS® 95 operating system fromMICROSOFT and comprising, apart from the sound card 25 a microprocessor27, an input/output interface 29, a controller 31 for mass storage 33and a video interface 35 linked by one or several bus 37. Thedigital-analog converter 41 of the sound card 25 comprises an outputterminal 8. The output terminal 8 of the sound card of the microcomputer14 is linked to the input terminal 8′ of the emitter 4, via an amplifier15 whose specifications are the following: passband from 10 Hz to 20kHz, gain 1 to 10, input sensitivity +/−1 V. Among the sound cards 25which can be used, one can cite, for example the Soundblaster 16 cardsold by the CREATIVELABS Company.

The transducer 6, situated inside the chamber 2, receives the fieldsresulting from the interaction between said specific excitation fieldand said substance 1. The transducer 6 transforms said resulting fieldsinto electric signals. These electric signals arrive at the outputterminals 9′ of the transducer 6 under the form of a variable differenceof potential or of an electric current of variable intensity. Thetransducer 6 comprises a bobbin with a soft iron core. This bobbin hasan impedance of 300 ohms, an internal diameter of 6 mm, an externaldiameter of 16 mm, and a length of 6 mm. The magnetic core in soft ironis placed in contact with the external walls of the container 3.

Advantageously, the characteristic electric signals available at theoutput from the transducer 6 are amplified by a preamplifier 16. Theamplifier-preamplifier 16 has the following specifications: passbandfrom 10 Hz to 20 kHz, gain 50 to 100 for an input sensitivity of +/−100mV or gain 500 to 2000 for an input sensitivity of +/−5 mV (to be usedin the case of an “opposition series” connection of a secondtransducer). The characteristic electric signals can b e recorded 31,stored 33, transferred 11, 29, remotely by implementing technologies ofelectronics, computers and telecommunications known to those skilled inthe art.

The recording of characteristic electric signals, or that of electricsignals derived after amplification or processing, can be carried out inanalog by a signal recorder, in particular o n magnetic tape, adapted tothe frequencies of the characteristic electric signals at the outputfrom the transducer 6. Since the passband used corresponds to the audioband, one can i n particular use a tape recorder. The output terminal 9′of the device for producing signals 10 is linked to the microphone inputor to the line input of such a tape recorder. During play, thecharacteristic electric signals recorded are collected at an outputterminal, in particular at the line output or at the loudspeaker outputof the tape recorder. Preferably, digital recording of thecharacteristic electric signals is carried out after analog-digitalconversion of said signals. In order to d o this, a micro-computer 17 isused, provided with a signal acquisition card 25. For example, one canuse a PC 17 type computer, operating on the WINDOWS® 95 operating systemfrom MICROSOFT. This microcomputer can be of the same type as that usedto generate the excitation field. It can be the same microcomputer. Inthis case it comprises, apart from the sound card, an acquisition card25, a microprocessor 27, an input/output interface 29, a controller 31,a mass storage 33 and a video interface 35 linked by one or several bus37. The acquisition card 25 comprises a analog-digital converter 39possessing, preferably, a resolution higher than 12 bits, andadvantageously equal to 16 bits, as well as a sampling frequency doublethe maximum frequency one wishes to be able to digitise, for example 44kHz. The output 9′ of the transducer 6 is linked to the input 9 of thedigital-analog converter 39 via the preamplifier 16.

All links consist of shielded cable. All the apparatus is earthed.

Advantageously, in order to process the characteristic electric signalsor the signal derivatives, one uses the Matlab software from the company“The MathWorks”. The output of the device 10 for producingcharacteristic electric signals is connected to the input 9 of theanalog-digital converter 39 of the card 25 of the computer 17. Oneproceeds with a n acquisition of characteristic electric signals for alength of time for example of between 1 and 60 sec (for example 6 sec)and the digital file is saved in a mass storage 33, for example underthe form of a sound file with the WAV format. This file can laterundergo digital processing, as for example digital amplification forcalibrating the signal level, filtering for eliminating unwantedfrequencies, or be transformed into its spectrum by a discrete FOURIERtransform, preferable by the algorithm of FFT “Fast Fourier Transform”.

The time length of the signal produced can be increased by repeatingseveral times in a file a fragment or the totality of the sound fileoriginally produced.

These processing means of characteristic electric signals can be used toimprove performances of said characteristic electric signals. In thecase of a first embodiment variant, a second transducer of the firsttype described above is envisaged. This second transducer transforms theexcitation field into electric signals, in the absence of saidsubstance. These electric signals are subtracted by an opposition seriesconnection to the signals derived from the first transducer. Thus oneobtains signals more representative of the interaction between thespecific excitation field and the substance. In the case of a secondembodiment variant, the processing means take into account thecharacteristics of the specific excitation field and reprocess thecharacteristic electric signals in the following way. First of all oneproceeds by calculating the spread of the PSD. Then this power spectraldensity is contracted by conserving only the frequency band ranging forexample from 140 Hz to 14 kHz, and reconstituting a signal from this PSDand randomly generated phases, and finally calibrating the power of thesignal thus produced.

The characteristic electric signals available at the exit of the outputfrom the device constituted by the combination of the emitter 4, thetransducer 6 and if applicable the preamplifier 16 already themselvesconstitute products suitable for industrial applications. They can beamplified, processed, saved, stored, transferred remotely byimplementing state of the art technologies in electronics, computers andtelecommunications. The industrial applications for which they can inparticular be implemented have been noted.

The file of characteristic electric signals, recorded under digital formas has just been described, possibly after processing, can betransferred remotely by a computer communication network. This networkcan comprise radio links 11. The file of characteristic electric signalsthus transmitted is recorded by the mass storage of the microcomputer18. For example, one can use a computer of the PC type, operating on aWINDOWS® 95 operating system from MICROSOFT. This microcomputer 18 canbe of the same type as that used for generating the excitation field.The file of characteristic signals thus transmitted and recorded can beexploited, in known ways, to produce analog characteristic electricsignals. The possibly processed file is transformed by a digital-analogconverter 41 of the card 25 (or a separate card) of the computer 18. Thedigital-analog converter 41 delivers analog electric signals to itsoutput 8 characteristic of the biological activity of the substance fromwhich they are issued. These signals can be transformed, as describedbelow, into electromagnetic fields and applied to biological systems.

Referring to FIG. 1c, a description is given of an embodiment of asystem making it possible to apply characteristic electric signals to abiological system receiver and to modify its chemical behaviour. Theflask 50 contains the biological system receiver. This is constituted,for example, of 10 ml distilled water for an injectable preparation(Biosédra or other brands) in a 15 ml tube in polypropylene (Falcon,Becton Dickinson 2097). This flask is set in an electromagnetic fieldradiated by a transducer 51, typically a bobbin. The bobbin, forexample, has a length of 120 mm, an internal diameter of 25 mm, anexternal diameter of 28 mm, with 631 turns of wire of 0.5 mm diameterand a resistance of 4 ohms. The bobbin 51 is earthed. Without thisrepresenting any limiting character, the bobbin 51 of the transducer hasa vertical axis making it possible to introduce the flask 50 containingthe receptor biological system. The input terminals 8′ of this bobbin 51are linked, in the case of the embodiment variant described, to theoutput 8 of the digital-analog converter 41 of the microcomputer 18 viaan amplifier 19 with the following specifications: passband from 10 Hzto 20 kHz, gain 1 to 20, input sensitivity 250 mV, output power RMS 60 Wunder 8 ohms, signal to noise ratio 80 dB. The voltage at the terminalsof the bobbin 51 has an amplitude of 5 Veff and the signal is appliedfor 10 minutes. The input terminals 8′ of the applicator can also be, inthe case of certain embodiment variants, directly connected to theoutput of the preamplifier 16 or to the output 8 of the digital-analogconverter 41 of the computer 17.

The invention also relates to the methods making i t possible to controlthe correlation between on the one hand, said signal derived from thetransducer 6 and on the other hand, the biological and/or chemicalactivity or the biological and/or chemical behaviour of said substanceor said active element contained in said substance. This control iscarried out by applying, by means of a transducer of the type describedin reference to FIG. 1c, signals derived from the transducer 6 to abiological control system and verifying that said biological controlsystem reacts in a specific manner to the signals derived from saidfirst transducer. In the case where said signals are processed, it isthese processed signals which are applied to said biological controlsystem. The reaction of said biological control system must be inrelation to the nature of the biological and/or chemical activity or thebiological and/or chemical behaviour of said substance or said activeelement contained in said substance from which are issued the signalsderived from said first transducer.

As an example of a biological control system, an d referring to FIG. 2,a test will be described below derived from that known under the testname of a perfused isolated guinea-pig heart (or Langendorff experiment)and whose process is described in the work entitled: “Methods inImmunology and Immunochemistry” published by Williams and Chase,Academic Press 1976, particularly page 68; or further in the workentitled: “L'experimentation animate en cardiologie” INSERMMédecine-Science—Coll. Flammarion—Author BernardSWYNGHEDAUW—particularly Ch. 3.1 p.81 “Organe Isolé—Coceur Isolé selonLangendorff—Montage à pression coronaire constante”; or further in thework entitled “The isolated perfused Heart according to Langendorff” H.J. Döiring, H. Dehnart—Biomesstechnik—Verlag March GmbH, D-7806 March.In FIG. 2 one recognises the diagram known from the Langendorffexperiment. The equipment described in these works has been completed bya transducer in the form of a bobbin 60 of a varnished copper wire ofdiameter 0.5 mm, with a diameter of 110 mm, a length of 40 mm and withan impedance of 4 ohms.

Three experiments were carried out with characteristic electric signalscoming respectively from the following substances:

for the first, ionophoretic-calcium A 23187 (Sigma C-7522) (I) at aconcentration of 10⁻⁶M in distilled water for injectable preparation(for example the Biosedra brand).

for the second, distilled water for injectable preparation (for examplethe Biosedra brand). (E)

for the third, caffeine (Sigma C-0750) (C) at a concentration of 10⁻⁶Min distilled water for injectable preparation (for example the Biosédrabrand). (E)

For each of these three experiments, the substances were placed in thecontainer 3 of the chamber 2 and their characteristic electric signalswere acquired in conformity with the operating process described withreference to FIGS. 1, 1 a and 1 b.

The three characteristic electric signals produced as described abovewere applied to the guinea-pig heart, connecting the terminals 8′ of thebobbin 60 to the output of the amplifier 19 of power 60 W. The threecharacteristic electric signals were applied for 2 minutes under avoltage of 5 Veff.

The fraction collector collected the tubes making it possible to measurethe debit of the guinea-pig heart at the rate of 1 tube per minute. Thebuffer solution crossing the heart had the following composition: CaCl 2mM, NaHCO3 25 mM, NaCl 118 mM, MgSO4 1.2 mM, KHPO4 1.2 mM, Glucose 11mM, Pyruvate 2 mM.

The table below shows (in ml) the quantity of the buffer solutionrecuperated in the collector tubes during the time.

Signal Time ionophoretic- Signal Signal mins. No signal calcium watercaffeine 1 4.4 4.4 4.5 4.3 2 4.3 4.3 4.5 4.4 3 4.3 4.4 4.4 4.4 4 4.4 4.34.5 4.5 5 4.4 4.2 4.5 4.2 6 4.3 4.9 4.4 4.0 7 4.3 5.2 4.4 3.6 8 4.4 5.44.5 3.4 9 4.3 5.4 4.5 3.2 10 4.4 5.2 4.4 3.0 11 4.3 5.0 4.5 3.0 12 4.45.0 4.4 3.2 13 4.3 4.8 4.4 3.4 14 4.4 4.8 4.5 3.6 15 4.3 4.6 4.4 3.8 204.3 4.5 4.4 4.0 25 4.3 4.5 4.5 4.1 30 4.3 4.5 4.4 4.0

This table shows that the guinea-pig heart reacted to the characteristicelectric signals coming from ionophoretic-calcium, water and caffeine asit would have reacted to injections of each of these three substances(see table below).

Time ionophoretic-calcium caffeine minutes Water 10⁻⁶M 10⁻⁶M 1 5.2 5.15.1 2 5.1 5.0 5.0 3 5.0 5.2 5.0 4 5.1 5.0 4.9 5 5.1 4.9 4.6 6 5.2 5.44.2 7 5.2 5.6 4.0 8 5.1 6.2 4.1 9 5.1 6.4 4.0 10 5.2 6.4 4.2 11 5.1 6.24.1 12 5.0 6.0 4.3 13 5.1 6.0 4.4 14 5.0 5.9 4.5 15 5.0 6.0 4.5 20 5.15.7 4.6 25 5.0 5.4 4.5 30 5.0 5.2 4.5

Next, as an example, a description follows with reference to FIGS. 3, 3a, 3 c and 3 d of a precipitation test between the polysaccharidicantigen of Escherichia coli K1 and an antibody against this antigenmaking it possible to control the characteristic electric signals of thebiological activity of Escheria coli. This test is defined below underthe name of precipitation test.

One tests the effects on a precipitation reaction between thepolysaccharidic antigen of Escherichia coli K1 and an antibody directedagainst this antigen:

from the application of a characteristic electric signal of thebiological activity of an antigenic substance foreign to this reactionsuch as the Streptococcus,

from the application of a characteristic electric signal of thebiological activity of the polysaccharidic antigen of Escherichia coli,

from the simultaneous application of a characteristic electric signal ofthe biological activity of Streptococcus and the characteristic electricsignal of the biological activity of an antibody directed againstEscherichia coli,

from the simultaneous application of a characteristic electric signal ofthe biological activity of Escherichia coli and the characteristicelectric signal of the biological activity of a n antibody directedagainst this antigen.

The acquisition of the characteristic electric signals of the biologicalactivities of Escherichia coli, of its specific antibody and of thepolysaccharidic antigen of Streptococcus was carried out by means of thedevice 10 described with reference to FIGS. 1, 1 a, 1 b.

The acquisition of the characteristic electric signal of the biologicalactivity of Streptococcus was carried out by placing at the centre ofthe chamber 2 a container 3 holding 1 ml of an aqueous suspension ofStreptococcus bacteria previously formalised (6.10⁶ cfu/ml).

The acquisition of the characteristic electric signals of the biologicalactivity of the specific antibody of Escherichia coli and its specificantibody was carried out by operating in the same manner, but usingrespectively:

a container 3 holding 1 ml of an aqueous suspension of bacteria ofEscherichia coli K1 previously formalised (6.10⁶ cfu/ml).

a container 3 holding 1 ml of a suspension of particles of a latexsensitised by a mouse monoclonal antibody specific of Escherichia coliK1, coming from a PASTOREX® MENINGITIS kit (Ref. 61709—SANOFIDIAGNOSTICS PASTEUR).

The tests were carried out using as reagents:

on the one hand, a solution of polysaccharidic antigen of Escherichiacoli K1 prepared by dissolving an antigenic extract from a PASTOREX®MENINGITIS kit (Ref. 61709—SANOFI DIAGNOSTICS PASTEUR) in 1 ml ofdistilled and sterile water, then dilution to 1/7, 1/7.5 or 1/8 inphysiological serum; and

on the other hand the latex sensitised by a mouse monoclonal antibodyspecific of Escherichia coli K1 present in this same kit, after dilutionto 1/3 in physiological serum.

For each of these tests, the following protocol was used:

one places in an oven heated to 37° C. a transducer 151 constituted by abobbin measuring 120 mm in length and 25 mm internal diameter, with 631turns and a resistance of 4.7 ohms and linked by its input terminal 8′to the output 8 of the digital-analog converter of a Soundblaster cardand a computer 17 (one could also use a computer 18 remotely)reinserting the recorded files constituted by the electric signals onewishes to apply for the time required to bring this transducer to thetemperature of 37° C.;

one deposits on a slide 147 supplied with a capillary 149 in aserpentine shape (of the type of those provided in the PASTOREXMENINGITIS kits), at a small distance from the opening of the latter, adrop 145 (40 to 50 μl) of the antigenic solution as described in pointb) above, together with a drop 143 (also corresponding to a volume of 40to 50 μl), latex sensitised by the antibody, taking care that thesedrops do not mix.

one applies, to the two drops of reagents thus deposited, the electricsignal or signals desired by placing the slide at the centre of thetransducer 151 for about 2 minutes and reinserting a sound file with theaid of the computer 17 (or the remote computer 18),

one mixes the two drops of reagents 143, 145 for about 10 seconds andthen leaves the reaction mixture in the oven for about 13 minutes tomigrate into the capillary and the precipitation reaction to take place:

one takes the blade out of the oven and then proceeds to read thisprecipitation.

This reading is carried out by analysis, by means of analysis softwareand image processing on a PC type computer using the WINDOWS® 95operating system (MICROSOFT), of an image acquired with the aid of avideo camera positioned on an optical microscope and connected to saidcomputer by a video acquisition card. The camera works in the greyshades. A first processing increases the contrast, the threshold beingset so that the precipitates appear in black, while the zones withoutlatex particles or precipitates appear white.

Based on the analysis of two-dimensional space spread of the dark zonesof the image, the computer determines a precipitation index (I)calculated according to the formula:$I = \frac{{{Surface}\quad {area}\quad {of}\quad {precipitates}\quad {of}\quad {size}} > {60\quad {pixels}}}{{{Surface}\quad {area}\quad {of}\quad {precipitates}\quad {of}\quad {size}} = {60\quad {pixels}}}$

The precipitation index is accordingly higher when the size of theprecipitates formed during the precipitation reaction is greater. Thecontrol test for the presence of a characteristic signal of thebiological activity of Escherichia coli is considered as positive when,during an experiment, the application of characteristic electric signalsof the biological activity of Escherichia coli and/or the biologicalactivity of its specific antibody leads to obtaining a precipitationindex significantly higher (by at least 40%) than the maximum of thoseobtained, under the same conditions, and over for example 3 experiments,after application of the characteristic electric signal of thebiological activity of Streptococcus.

Table A below shows the precipitation indexes obtained in a first seriesof tests aimed at comparing the effects of the application ofcharacteristic electric signals of the biological activity ofEscherichia coli (E. coli) coming from a biological system containingEscherichia coli with those observed after application, under the samereaction conditions, of characteristic electric signals of thebiological activity of Streptococcus (St) coming from a biologicalsystem containing Streptococcus and for 3 different dilutions (1/7,1/7.5 and 1/8) of the polysaccharidic antigen of Escherichia coli K1used as reagent in the precipitation reactions.

TABLE A Dilution of the solution of Precipitation index (I) E. coli K1antigen Signal St Signal E. coli 1/7 11 173 6 52 16 154 1/7.5 58 141 32117 12 107 1/8 10 113 6 37 8 21

Moreover, FIGS. 3a and 3 b show, as examples, images of the precipitatesformed, on the one hand, after application of the characteristicelectric signal of the biological activity of Streptococcus (FIG. 3a)and, on the other hand, after application of the characteristic electricsignal of the biological activity of Escherichia coli (FIG. 3b). Theseimages correspond respectively to the precipitation indexes of 32 and117 which are recorded on line 5 of Table A.

As for Table B below, the precipitation indexes obtained in a secondseries of experiments within the framework of which the effects ofsimultaneous application of the characteristic electric signal of thebiological activity of Escherichia coli and the characteristic electricsignal of the biological activity of the antibody directed againstEscherichia coli were compared to those of the simultaneous application,under the same reaction conditions, of the characteristic electricsignal of the biological activity of Streptococcus and of thecharacteristic electric signal of the biological activity of theantibody directed against Escherichia coli, carried out for 2 differentdilutions (1/7 and 1/7.5) of the polysaccharidic antigen of Escherichiacoli K1 used as reagent.

TABLE B Precipitation index (I) Dilution of the Signal St + Signal E.coli + solution of Signal antibody Signal antibody E. coli K1 antigenanti-E. coli anti-E. coli 1/7 18 94 71 247 1/7.5 48 212 93 1141

FIGS. 3c and 3 d show, also as examples, images of precipitatescorresponding respectively to the precipitation indexes 71 and 247recorded on line 2 of Table B.

All these results demonstrate clearly the aptitude presented by aligand/receptor couple for revealing and controlling the presence of acharacteristic electric signal of the biological activity of a ligandand/or its receptor. In fact, in the presence of a specificcharacteristic signal of the ligand/receptor couple or one of theelements of this couple, the formation of complexes formed by thereaction between this ligand and this receptor is amplified. Thisamplification is very specific, since the characteristic electric signalof the biological activity of a biologically active element, but foreignto this reaction, does not itself produce this amplification effect.

In the meaning of the present invention, the “ligand/ receptor couple”means any couple formed by two substances able to recognise each otherspecifically, to link together and to act together to form complexes.Thus, it can concern an antigen/antibody couple, or hapten/antibody inwhich the ligand (the antigen or the hapten) can be a biologicalcompound (protein, enzyme, hormone, toxin, tumour tag), a chemicalcompound (toxic or medicated active principle, for example), or a cellor particle antigen (cell, bacteria, virus, fungus, . . . ), thereceptor being able to be a soluble antibody or a membranous receptor.It can also be a couple formed by an enzyme and its specific substrate.

These results show clearly that it is possible to use ligand/receptorcouples and, in general, test biological systems to constitute acorrelation table between the characteristic signals issued from adetermined substance or from an active element contained in a determinedsubstance and the modifications they can induce on test biologicalsystems, in particular such as a ligand/receptor couple.

These correlation tables can be used later for detecting active elementsby analysing the effects of characteristic signals coming from them ontest biological systems recorded in the correlation table.

As an example, with reference to FIG. 4, a presentation is given belowof the test known under the name of guinea-pig cutaneous test anddescribed in chapter 11 (p.346-351) in the second edition of“Immunology” edited by Jean-Francois Bach, coll. John Wiley & Sons; orfurther in the 3rd edition of “The handbook of Experimental Immunology”edited by D. M. Weir, coll. Blackwell, Ch. 21 “Passive cutaneousanaphylaxis (PCA)” by W. E. Brocklehurst; or further in the work editedby Williams & Chase entitled “Methods in IMMUNOLOGY andIMMUNOCHEMISTRY”—Vol. 5—Ch. 19 “Anaphylaxis”.

The guinea-pig is used when still alive, and is given a n intravenousinjection of a blue colorant (Evans blue—Sigma E 2129) which fixes onthe blood albumin. The albumin does not leave the vessels, unless thereis inflammation, and thus vasodilatation and permeability of thevessels, the typical example of such a reaction with man beingurticaria.

The test is carried out by injecting under the skin of the animalprepared in this way, 0.1 ml of the solution whose activity is to becontrolled. Nextone measures the diameter of the blue marks appearingaround the points of injection. In order to do this the skin is scanned,and then the bitmap image file is recorded. Finally the sizes of theblue marks due to the reaction are evaluated.

In the example described, a control was carried out of the presence ofsignals characteristic of the biological activity of the acetylcholineneuromediator (ACh; Sigma A2661) in solution in a physiologicalsolution, by analysing the effects o n the skin of a guinea-pig:

on the one hand, of an injection of 0.1 ml distilled water, afterapplying to this distilled water a characteristic electric signal of thebiological activity of acetylcholine,

on the other hand, of an injection of 0.1 ml distilled water, afterapplying to this distilled water a characteristic electric signal of thebiological activity of a product close to acetylcholine but inactive:the mixture acetate/choline (A-C) (A: Sigma S8625; C: Sigma C7017).

The acquisition of the characteristic electric signals of the biologicalactivities of acetylcholine and the acetate/choline mixture was carriedout by means of the device 10 described with reference to FIGS. 1, 1 a,1 b.

The acquisition of the characteristic electric signal of the biologicalactivity of acetylcholine was carried out by placing in the centre ofthe chamber 2 a container 3 holding 1 ml of a solution of acetylcholinein distilled water at the concentration of 10⁻⁶M.

The acquisition of characteristic electric signals of the biologicalactivity of the mixture acetate/acetylcholine was carried out byoperating in the same manner, but using a container 3 holding 1 ml of asolution of acetate/acetylcholine in distilled water at theconcentration of 10⁻⁶M.

For each of the tests, the following protocol was used:

The bobbin 51 of FIG. 1c was used as applicator.

The numbers figuring in the first column of tables C, D and E belowcorrespond to the references in FIG. 4.

TABLE C No. Distilled water solution injected Dia. in mm 200 Afterapplication 12 201 of the ACh signal 6 202 7 203 7 204 16 300 Withoutapplication 3 301 of the signal 2 302 4 303 3 304 1 305 0 306 1

Experiments numbered 200 to 204 show that the solutions of distilledwater injected after application of the ACh signal setoff a significantcutaneous reaction (average 11 mm) compared with the same solutions ofdistilled water injected without application of the ACh signal. Thelatter d o not setoff a reaction as shown in experiments numbered 300 to306 (3 mm).

TABLE D No. Solution injected Dia. in mm 310 ACh in 10⁻⁶M solution 23311 25 312 23 313 21 314 18

Comparison of the experiments in tables C and D shows that theinjections of solutions of distilled water after application of the AChsignal (experiments 200 to 204) have effects which are less, butcomparable, on the guinea-pig skin to those of injections of ponderalACh solutions (experiments 310 to 314).

TABLE E No. Distilled water solution injected Dia. in mm 400 Afterapplication 2 401 of the A-C signal 2 402 1 403 3 404 1 410 A-C insolution at 10⁻⁶M 3 411 2 412 1

Experiments numbered 400 to 404 and 410 to 412 in Table E are carriedout from a product close to acetylcholine but inactive: theacetate/choline (A-C) mixture.

Experiments 410, 411, 412, correspond to an injection of a ponderalsolution of A-C 10⁻⁶M. One notes that an injection of distilled watersolution after application of the A-C signal (exp. 400 to 404) and thata ponderal injection (exp. 410, 411, 412) do not provoke any effect(diameter between 1 and 3 mm). These injections show that the cutaneousreaction of the guinea-pig is really specific to the nature of thesubstance in solution because these injections, carried out under thesame conditions as the injections numbered 200 to 202, have no effect.

The experiments of tables C to E make it evident that the guinea-pigskin test makes it possible to control the presence of a signal comingfrom a substance with a biological activity such as acetylcholine.

Below is described the method used for controlling the followinghomeopathic products: arnica 7CH, acetylcholinum 7CH.

First of all one has to produce the characteristic signals of theproduct to be tested. In the case where the homeopathic product to testis a solution, one proceeds by registering a sample of 1 ml as describedin this patent. In the case where one wishes to test homeopathicgranules, first of all a solution is prepared, for example 5 ml, bydiluting 2 granules per ml of distilled water for injectable preparation(for example the Biosédra brand), and then one proceeds with theregistering of a sample of 1 ml according to the method described inthis patent.

Nextone uses for example one or several of the three methods describedabove (perfused isolated guinea-pig heart; precipitation test of aligand/receptor couple and cutaneous test on a guinea-pig). Since thecorrelation between the reaction of these biological control systems andthe biological and/or chemical activity of the product having served toproduce the homeopathic product has been demonstrated, a positivereaction of the biological control system will show the presence of theactivity searched for in the homeopathic product tested. In the sameway, a negative reaction of the biological control system will show theabsence of the activity searched for in the homeopathic product tested.

Result on the skin of a Product to be guinea-pig Detection of tested(diameter of marks in mm) activity Neutral granules  0.6 ± 0.5 (n = 5)NO Arnica 7CH 16.6 ± 2.9 (n = 5) YES granules

What is claimed is:
 1. Method for producing from a substance (1)signals, in particular electric signals, characteristic of thebiological and/or chemical activity or of the biological and/or chemicalbehaviour of said substance or of an active element contained in saidsubstance; said method comprising the stages: of placing said substancein a zone (2) submitted to a specific excitation field of electric,magnetic and/or electromagnetic nature (4), said specific excitationfield having the characteristic of having a power spectral densityspread over a frequency band, particularly of the white noise type or ofthe pink noise type; said method also comprising: of transforming theresulting fields from the interaction of the specific excitation fieldof the substance into signals, in particular electric signals, by meansof a first transducer (6) receiving said resulting fields, said signalsbeing characteristic of the biological and/or chemical activity or ofthe biological and/or chemical behaviour of said substance or saidactive element contained in said substance.
 2. Method according to claim1 further comprising the stage processing (17) said signals derived fromsaid first transducer (6) in function of second signals derived from asecond transducer receiving the specific excitation field, in theabsence of said substance or an active element contained in saidsubstance, in such a way that said processed signals are advantageouslycharacteristic of the biological and/or chemical activity or of thebiological and/or chemical behaviour of said substance or said activeelement contained in said substance.
 3. Method according to claim 1further comprising the stage: of processing (17) the signals derivedfrom said first transducer, in function of the characteristics of thespecific excitation field, in such a way that said processed signals areadvantageously characteristic of the biological and/or chemical activityor of the biological and/or chemical behaviour of said substance or saidactive element contained in said substance.
 4. Method according to claim1, said specific excitation field having a power spectral density suchthat, in the absence of said substance or an active element contained insaid substance, the power spectral density of the signals produced bysaid first transducer of produced by an opposition series connection ofsaid first and second transducers is uniform.
 5. Method according toclaim 1, said specific excitation field being generated by a sinusoidalsignal generator of variable frequency with time and scanning afrequency band.
 6. Method according to claim 5, said frequency bandbeing in a frequency range lower than 100 kHz.
 7. Method according toclaim 1, said specific excitation field having the characteristic ofhaving a uniform power spectral density over a band of frequencies, insuch a way that said substance will be submitted to a neutral excitationfield of white noise type.
 8. Method according to claim 1, such that:the zone (2) submitted to the specific excitation field is isolated fromparasitic fields coming from the environment.
 9. Method according toclaim 1 further comprising the stage: of applying, by means of a thirdtransducer (51, 151) to a receptor biological system said signalsderived from said first transducer (6) or the processed signal, in sucha way that the biological and/or chemical activity or the biologicaland/or chemical behaviour of the receptor biological system will bemodified in function of the nature of the biological and/or chemicalactivity or the biological and/or chemical behaviour of said substance.10. Method according to claim 9, further comprising the stage: ofcontrolling the correlation between, on the one hand the signal derivedfrom said first transducer (6) or the processed signal, and on the otherhand the biological and/or chemical activity or the biological and/orchemical behaviour of said substance or said active element contained insaid substance, by applying, by means of said third transducer (51,151), the signal derived from the first transducer (6) or the processedsignal to a biological control system and verifying that said biologicalsystem reacts in a specific manner to the signal derived from said firsttransducer or to the processed signal, according to the nature of thebiological and/or chemical activity or the biological and/or chemicalbehaviour of said substance or said active element contained in saidsubstance from which is issued the signal derived from said firsttransducer or the processed signal.
 11. Method according to claim 10,such that the biological system is an isolated guinea-pig heart. 12.Method according to claim 10, such that the biological system is aligand/receptor couple particularly an antigen/antibody couple. 13.Method according to claim 10, such that the biological system is aninjectable substance provoking cutaneous reactions.
 14. Method accordingto claim 10, such that the biological system is composed of isolatedcells or cells in culture.
 15. Method according to claim 1, such thatsaid substance contains an active element in low or very lowconcentration.
 16. System for producing signals, particularly electricsignals, characteristic of the biological and/or chemical activity orthe biological and/or chemical behaviour of substance (1) or an activeelement contained in said substance and system for implementing theproperties of such signals, said system comprising: an emitter (4)generating a specific excitation field of electric, magnetic and/orelectromagnetic nature in a zone (2, 3) where said substance issituated; said specific excitation field having the characteristic ofhaving a power spectral density spread over a frequency band,particularly of the white noise type or of the pink noise type; saidmethod also comprising: a first transducer (6) receiving the resultingfields from the interaction of said specific excitation field and saidsubstance, said first transducer transforming said resulting fields intosignals, particularly electric signals, said signals beingcharacteristic of the biological and/or chemical activity or thebiological and/or chemical behaviour of said substance or said activeelement contained in said substance, means of emission (51, 151) forapplying said signals derived from said first transducer to a receptorbiological system, in such a way that the biological and/or chemicalactivity or the biological and/or chemical behaviour of the receptorbiological system will be modified in function of the nature of thebiological and/or chemical activity or the biological and/or chemicalbehaviour of said substance.
 17. System according to claim 16 furthercomprising: means (17) for processing said signals derived from saidfirst transducer (6), in function of the signals derived from a secondtransducer receiving the specific excitation field, in the absence ofsaid substance or an active element contained in said substance, so thatsaid processed signals are advantageously characteristic of thebiological and/or chemical activity or the biological and/or chemicalbehaviour of said substance or said active element contained in saidsubstance.
 18. System according to claim 16 further comprising: means(17) for processing the signals derived from said first transducer (6),in function of the characteristics of the specific excitation field, sothat said processed signals are advantageously characteristic of thebiological and/or chemical activity or the biological and/or chemicalbehaviour of said substance or said active element contained in saidsubstance.
 19. System according to claim 16 such that said emittergenerates a specific excitation field with a power spectral density suchthat, in the absence of said substance or an active element contained insaid substance, the power spectral density of the signals produced bysaid first transducer or produced by an opposition series connection ofsaid first and second transducers is uniform.
 20. System according toclaim 16 such that said emitter generating said specific excitationfield comprises a sinusoidal signal generator of frequency variable withtime and sweeping a frequency band.
 21. System according to claim 20,such that said frequency band is in a frequency range lower than 100kHz.
 22. System according to claim 16, said specific excitation fieldhaving the characteristic of having a uniform power spectral densityover a frequency band.
 23. System according to claim 16, such that itfurther comprises: means (2) for isolating said zone from parasiticfields from the environment.
 24. System according to claim 16, furthercomprising means of control for controlling the correlation between, onthe one hand, the signal derived from said first transducer or theprocessed signal and, on the other hand, the biological and/or chemicalactivity or the biological and/or chemical behaviour of said substanceor said active element contained in said substance, said means ofcontrol comprising a third transducer (51, 60, 151) applying the signalderived from said first transducer or the processed signal to abiological control system, said means of control further comprisingmeans for verifying that the biological control system reacts in aspecific manner to the signal derived from first transducer or theprocessed signal, according to the nature of the biological and/orchemical activity or the biological and/or chemical behaviour of saidsubstance or said active element contained in said substance from whichis issued the signal derived from said first transducer or saidprocessed signal.
 25. System according to claim 24, such that thebiological control system is an isolated guinea-pig heart (FIG. 2). 26.System according to claim 24, such that the biological control system isa ligand/receptor couple particularly an antigen/antibody couple (FIG.3).
 27. System according to claim 24, such that the biological controlsystem is an injectable substance provoking cutaneous reactions (FIG.4).
 28. System according to claim 24, such that the biological controlsystem is composed of isolated cells or cells in culture.
 29. Systemaccording to claim 16, such that said substance contains an activeelement in low or very low concentration.
 30. Device for producingsignals, particularly electric signals, characteristic of the biologicaland/or chemical activity or the biological and/or chemical behaviour ofa substance or an active element contained in said substance, saiddevice comprising: an emitter (4) generating a specific excitation fieldof electric, magnetic and/or electromagnetic nature in a zone where saidsubstance is situated, said specific excitation field having thecharacteristic of having a power spectral density spread over afrequency band, particularly of the white noise type or of the pinknoise type; said method also comprising: a first transducer (6)receiving the resulting fields from the interaction of said specificexcitation field and said substance, said first transducer transformingsaid resulting fields into signals, particularly electric signals, saidsignals being characteristic of the biological and/or chemical activityor the biological and/or chemical behaviour of said substance or saidactive element contained in said substance.
 31. Device according toclaim 30 further comprising: means (17) for processing the signalsderived from said first transducer, in function of the signals derivedfrom a second transducer receiving the specific excitation field, in theabsence of said substance, so that said processed signals areadvantageously characteristic of the biological and/or chemical activityor the biological and/or chemical behaviour of said substance or saidactive element contained in said substance.
 32. Device according toclaim 30 further comprising: means (17) for processing the signalsderived from said first transducer, in function of the characteristicsof the specific excitation field so that said processed signals areadvantageously characteristic of the biological and/or chemical activityor the biological and/or chemical behaviour of said substance or saidactive element contained in said substance.
 33. Device according toclaim 30 such that said emitter generates a specific excitation fieldwith a power spectral density such that, in the absence of saidsubstance or an active element contained in said substance, the powerspectral density of the signals produced by said first transducer orproduced by an opposition series connection of said first and secondtransducers is uniform.
 34. Device according to claim 30 such that saidemitter generating said specific excitation field comprises a sinusoidalsignal generator of frequency variable with time and sweeping afrequency band.
 35. Device according to claim 34, such that saidfrequency band is in a frequency range lower than 100 kHz.
 36. Deviceaccording to claim 30, said specific excitation field having thecharacteristic of having a uniform power spectral density over afrequency band.
 37. System according to claim 30, such that it furthercomprises: means (2) for isolating said zone from parasitic fields fromthe environment.
 38. Application of the method, system or deviceaccording to claim 1 to the production of active substances particularlyto the production of drugs; said active substances being produced byapplying said signals derived from said first transducer (6) or saidprocessed signals to a carrier substance.
 39. Application of the method,system or device according to claim 1 to establishing a correlationtable between the characteristic signals issued from a determinedsubstance or from an active element contained in said determinedsubstance and the modifications they can induce on test biologicalsystems.
 40. Correlation table established in conformity with claim 39.41. Utilisation of the correlation table according to claim 40, for thedetection of said determined substance or said active element containedin said determined substance, particularly remotely, after transmissionof said characteristic signal to a testing laboratory possessing saidtest biological systems.
 42. Utilisation of the correlation tableaccording to claim 41, to control the production of homeopathicproducts.
 43. Signal linked to a biological and/or chemical activity,obtained by means of a method according to claim
 1. 44. Signal linked toa biological and/or chemical activity, obtained by means of a systemaccording to claim
 16. 45. Signal linked to a biological and/or chemicalactivity, obtained by means of a device according to claim 30.